AKTIVITAS ANTIBAKTERI EKSTRAK DAUN BELIMBING WULUH (Averrhoa bilimbi L.) TERHADAP BAKTERI PATOGEN PANGAN

Authors

  • Lucia Crysanthy Soedirga Universitas Pelita Harapan

Keywords:

Antibacterial, bilimbi leaves, maceration, pathogenic bacteria, well diffusion

Abstract

Antibacterial activity of Averrhoa bilimbi leaves extracts on pathogenic bacteria was observed in this study by using well diffusion method. The leaves were extracted using maceration method with three different solvents, i.e. ethanol (polar), ethyl acetate (semi polar), and hexane (non polar). Moreover, different concentrations (5,10,15,20, and 25%) of bilimbi leaves extract were also observed in this study in order to determine which solvent and concentration gave the highest antibacterial activity against B.cereus, S.aureus, Pseudomonas sp., and Enterobacter sp. Further objectives of this research were to determine the MIC and MBC for each bacteria from the selected extract. The result showed that ethanol extract gave the highest antibacterial activity with inhibition diameter ranging from 2.55 mm for Enterobacter sp. to 12.36 mm for B.cereus. Moreover, MIC and MBC for Gram negative higher than the MIC and MBC for Gram positive.

Author Biography

Lucia Crysanthy Soedirga, Universitas Pelita Harapan

Food Technology Study Program

References

Andrews, J. M. 2001. Determination of minimum inhibitory concentrations. Journal of Antimicrobial Chemotherapy, 48(suppl_1), 5-16. DOI: 10.1093/jac/48.suppl_1.5. Murhadi, Suharyono, A.S., dan Susilawati. 2007. Aktivitas antibakteri ekstrak daun salam (Syzygium polyanta) dan daun pandan (Pandanus amaryllifolius).” Jurnal Teknologi dan Industri Pangan, Vol XVII, no.1. Madigan, M.T., Martinko, H.M., dan Parker, J. 2006. Brock Biology of Microorganisms. Southern Illinois: Prentice Hall. Nychas, G.J.E dan Tassou, C.C. 2000. Traditional Preservatives-Oil and Spices. Dalam: R.K. Robinson, C.A. Batt, P.D. Patel. Encyclopedia of Food Microbiology Volume 1. London: Academic Press. Okiei, W. O., Ogunlesi, M., Osibote, E. A., Binutu, M. K., dan Ademoye, M. A. 2011. Comparative studies of the antimicrobial activity of components of different polarities from the leaves of Nauclea latifolia. Sahgal, G., Sreeramanan, S., Sasidhran, S., Xavier, R., dan Ong, M.T. 2010. Screening selected medicinal plants for antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA). Adv.Nat.Appl. Sci, 3(3): 330-338. Zakaria, Z. A., Zaiton, H., Henie, E. F. P., Jais, A. M., and Zainuddin, E. N. 2007. In vitro antibacterial activity of Averrhoa bilimbi L. leaves and fruits extracts. International Journal of Tropical Medicine, 2(3), 96-100.

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Published

2019-11-29

Issue

Vol. 3 No. 2 (2019): NOVEMBER

Section

Articles

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