Pemanfaatan Kitosan Kepiting Bakau (Scylla serrata) dan Ion Ag+ dalam Pembuatan Lapisan Antibakteri pada Casing Handphone
DOI:
https://doi.org/10.19166/fastjst.v10i1.10886Keywords:
Ag+ ion, agar well diffusion, antibacterial, chitosan, Scylla serrataAbstract
Phone cases are essential for protecting mobile phones from dirt and the risk of damage. However, it may also serve as reservoirs for bacterial growth, including Staphylococcus aureus, due to frequent handling and environmental exposure. To address this issue, an antibacterial coating was developed using an epoxy resin matrix incorporated with chitosan derived from Scylla serrata and silver ions (Ag⁺) obtained from AgNO₃ as active antibacterial agents. This study aimed to evaluate the antibacterial effectiveness of Scylla serrata chitosan and Ag⁺ ions and to determine the optimal chitosan-to-Ag⁺ ratio for inhibiting Staphylococcus aureus growth on coated surfaces. An in vitro antibacterial assay was performed using the agar well diffusion method on Nutrient Agar by applying 75.36 µL of chitosan–AgNO₃ solutions at ratios of 0:1, 1:0, 1:1, 2:1, and 1:2, with epoxy resin in 99.9% absolute ethanol as the negative control and 100 ppm amoxicillin as the positive control. Antibacterial activity was assessed by measuring inhibition zone diameters, and data were analyzed using tests of normality and homogeneity followed by one-way ANOVA and the Games–Howell post hoc test. The results demonstrated that both chitosan and Ag⁺ ions exhibited significant antibacterial activity, with the highest inhibition observed for the 0:1 and 1:0 ratios, while the combined chitosan–Ag⁺ formulation at a 1:1 ratio showed the most effective synergistic antibacterial performance against Staphylococcus aureus.
References
[1] M. Gashaw, M. Abtew, and A. Addis, “Prevalence and antimicrobial susceptibility pattern of bacteria isolated from mobile phones of health care professionals working in Gondar town health centers,” Journal of Environmental and Public Health, vol. 2014, Art. no. 205074, 2014. doi: 10.1155/2014/205074.
[2] M. Potara, E. Jakab, A. Damert, O. Popescu, V. Canpean, and S. Astilean, “Synergistic antibacterial activity of chitosan–silver nanocomposites on Staphylococcus aureus,” Nanotechnology, vol. 22, no. 13, p. 135101, 2011. doi: 10.1088/0957-4484/22/13/135101.
[3] F. Nurainy, S. Rizal, and Y. Yudiantoro, “Pengaruh konsentrasi kitosan terhadap aktivitas antibakteri dengan metode difusi agar (sumur),” Jurnal Teknologi Industri dan Hasil Pertanian, vol. 13, no. 2, pp. 117–123, 2008.
[4] A. Rahman, I. Hardi, and A. Baharuddin, “Identifikasi bakteri Staphylococcus sp. pada handphone dan analisis praktik personal hygiene,” Window of Health, vol. 1, no. 1, pp. 40–47, 2018.
[5] M. U. E. Sanam, A. I. R. Detha, and N. K. Rohi, “Detection of antibacterial activity of lactic acid bacteria isolated from Sumba mare’s milk against Bacillus cereus, Staphylococcus aureus, and Escherichia coli,” Journal of Advanced Veterinary and Animal Research, vol. 9, no. 1, p. 53, 2022. doi: 10.5455/javar.2022.i568.
[6] C. Wendakoon, P. Calderon, and D. Gagnon, “Evaluation of selected medicinal plants extracted in different ethanol concentrations for antibacterial activity against human pathogens,” Journal of Medicinally Active Plants, vol. 1, no. 2, pp. 60–68, 2012.
[7] C.-J. Yao, S.-J. Yang, M.-J. Shieh, and T.-H. Young, “Development of a chitosan–silver nanocomposite/β-1,3-glucan/hyaluronic acid composite as an antimicrobial system for wound healing,” Polymers, vol. 17, no. 3, p. 350, 2025. doi: 10.3390/polym17030350.
[8] J. Florencia, “Menilik manfaat ion perak dalam membunuh bakteri,” KlikDokter, 2017. [Online]. Available: https://www.klikdokter.com
[9] M. Ambari, “Kepiting dan rajungan: Enak rasanya, unggulan ekspor Indonesia, bagaimana keberlanjutannya?” Mongabay Indonesia, 2024. [Online]. Available: https://mongabay.co.id
[10] R. Riski and F. J. Sami, “Formulasi krim anti jerawat dari nanopartikel kitosan cangkang udang windu (Penaeus monodon),” Jurnal Farmasi UIN Alauddin Makassar, vol. 3, no. 4, pp. 1–6, 2017.
[11] G. E. C. Alouw, Fatimawali, and J. S. Lebang, “Uji aktivitas antibakteri ekstrak etanol daun kersen (Muntingia calabura L.) terhadap bakteri Staphylococcus aureus dan Pseudomonas aeruginosa dengan metode difusi sumuran,” Pharmacy Medical Journal, vol. 5, no. 1, pp. 36–45, 2022, doi: 10.35799/pmj.v5i1.41430
[12] N. B. A. Prasetya, Ngadiwiyana, Ismiyarto, and P. R. Sarjono, “Synthesis and study of antibacterial activity of polyeugenol,” IOP Conference Series: Materials Science and Engineering, vol. 509, no. 1, Art. no. 012101, 2019, doi: 10.1088/1757-899X/509/1/012101.
[13] A. W. Lestari, Z. Marlita, V. Sefiya, and I. A. Prasetyo, “Analysis of variance (ANOVA): Concept, steps, and its application in data analysis,” J. Sintesis, vol. 6, no. 1, 2025, doi: 10.56399/jst.v6i1.283.
[14] T. Sadiq, A. Arikan, T. Sanlidag, E. Guler, and K. Suer, “Big concern for public health: Microbial contamination of mobile phones,” Journal of Infection in Developing Countries, vol. 15, no. 6, pp. 798–804, 2021, doi: 10.3855/jidc.13708.
[15] S. S. M. Talabani and A. Taifor, “Simulation the effect of impact on the mobile cover using SolidWorks analysis,” ZANCO Journal of Pure and Applied Sciences, vol. 31, no. s3, 2019, doi: 10.21271/ZJPAS.31.s3.6.
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