Pemanfaatan Kitosan Kepiting Bakau (Scylla serrata) dan Ion Ag+ dalam Pembuatan Lapisan Antibakteri pada Casing Handphone
DOI:
https://doi.org/10.19166/fastjst.v10i1.10886Keywords:
Ag+ ion, antibakteri, difusi agar sumur, kitosan, Scylla serrataAbstract
Casing handphone berfungsi melindungi handphone dari kotoran dan risiko kerusakan, namun juga berpotensi menjadi media pertumbuhan bakteri seperti Staphylococcus aureus. Untuk mengatasi masalah tersebut, dikembangkan lapisan antibakteri pada casing handphone yang terdiri dari epoxy resin dengan kombinasi kitosan Scylla serrata dan ion Ag⁺ dari AgNO₃ sebagai agen antibakteri. Penelitian ini bertujuan untuk mengetahui efektivitas kitosan Scylla serrata dan ion Ag⁺ dalam lapisan antibakteri serta menentukan rasio perbandingan terbaik dalam menghambat pertumbuhan Staphylococcus aureus. Penelitian dilakukan secara in vitro menggunakan metode difusi agar sumuran pada media Nutrient Agar. Larutan kitosan–AgNO₃ sebanyak 75 µL dengan rasio (C:Ag⁺) 0:1, 1:0, 1:1, 2:1, dan 1:2 diuji terhadap bakteri. Epoxy resin dengan etanol absolut 99,9% digunakan sebagai kontrol negatif, sedangkan amoksisilin 100 ppm sebagai kontrol positif. Zona hambat diukur dalam satuan milimeter dan dianalisis menggunakan uji normalitas, homogenitas, Oneway ANOVA, dan Games-Howell. Hasil menunjukkan bahwa rasio 0:1 dan 1:0 memiliki daya hambat tertinggi, sedangkan kombinasi kitosan–Ag⁺ paling efektif pada rasio 1:1.
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