PRODUKSI N-ASETILGLUKOSAMIN DARI KITIN KULIT UDANG DENGAN FERMENTASI MENGGUNAKAN Salmonella enterica STRAIN LT2 [PRODUCTION OF N-ACETYLGLUCOSAMINE FROM SHRIMP SHELLS’ CHITIN BY FERMENTATION USING Salmonella enterica STRAIN LT2]

Authors

  • Yuniwaty Halim Universitas Pelita Harapan
  • Lucia Crysanthy Soedirga Universitas Pelita Harapan
  • Valentina Michelle Universitas Pelita Harapan

DOI:

https://doi.org/10.19166/jstfast.v6i2.5920

Keywords:

chitin, fermentation, microorganism, P. monodon, S. enterica

Abstract

Chitin is found abundantly in shrimp shells and can be converted into N-acetylglucosamine, which has a wide range of uses in the biomedical and industrial fields. Chitin can be produced using chitinase produced by bacteria through the fermentation process. Salmonella enterica strain LT2 was one of the chitinolytic bacteria that was isolated from shrimp shells. This research aimed to determine the best pH (5, 6, 7, 8, and 9) and temperature (32oC, 37oC, and 42oC) of fermentation, as well as fermentation time (2, 3, 4, 5, and 6 days) for the production of N-acetylglucosamine using the S. enterica strain LT2. Results showed that the highest production of N-acetylglucosamine occurred at the temperature of 37oC, pH of 8, and 4 days of fermentation, which produced 69.62 ± 1.00 g/L of N-acetylglucosamine. Furthermore, the highest N-acetylglucosamine production for the fermentation time occurred on the third day which produced 73.19 ± 1.63 g/L of N-acetylglucosamine.

Bahasa Indonesia Abstract:

Kitin banyak ditemukan pada cangkang udang dan dapat dipecah menjadi N-asetilglukosamin, yang banyak digunakan dalam dunia biomedik maupun industri lainnya. Kitin dapat diproduksi menggunakan kitinase yang dihasilkan oleh bakteri melalui proses fermentasi. Salmonella enterica strain LT2 merupakan salah satu bakteri kitinolitik yang diisolasi dari cangkang udang. Penelitian ini bertujuan untuk menentukan pH (5, 6, 7, 8, dan 9) dan suhu fermentasi (32oC, 37oC, dan 42oC) terbaik, serta lama fermentasi terbaik (2, 3, 4, 5, dan 6 hari) untuk produksi N-asetilglukosamin menggunakan S. enterica LT12. Hasil penelitian menunjukkan bahwa produksi N-asetilglukosamin diperoleh pada suhu fermentasi 37oC, pH 8, dan waktu fermentasi 4 hari, yang menghasilkan N-asetilglukosamin sebesar 69,62 ± 1,00 g/L. Selain itu, produksi N-asetilglukosamin tertinggi terjadi setelah fermentasi pada hari ketiga, yaitu sebesar 73,19 ± 1,63 g/L.


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Published

2022-11-30

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Vol. 6 No. 2 (2022): NOVEMBER

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