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Email this article IMOBILISASI KITINASE INTRASELULER Providencia stuartii DENGAN KALSIUM ALGINAT DAN APLIKASINYA DALAM PRODUKSI N-ASETILGLUKOSAMIN
Abstract
Kitin, polimer linier yang terdiri dari unit ß-1,4-N-asetilglukosamin, ditemukan secara alami pada cangkang udang dan dapat diubah menjadi glukosamin, yang memiliki fungsi yang luas, khususnya di bidang kesehatan untuk mengobati penyakit pada sendi. N-asetilglukosamin (NAG), salah satu bentuk glukosamin, dapat dihasilkan melalui fermentasi kitin menggunakan mikroorganisme kitinolitik seperti kapang atau bakteri. Produksi kitinase oleh Providencia stuartii telah dipelajari, namun imobilisasi kitinase untuk produksi NAG belum secara langsung dievaluasi. Penelitian ini bertujuan untuk menentukan pengaruh rasio antara kitinase intraselular dan support menggunakan alginat dan pengaruh banyaknya siklus fermentasi terhadap aktivitas enzim kitinase intraseluler yang diimobilisasi dan produksi NAG dari kitin yang diperoleh dari cangkang udang Penaeus monodon. Rasio kitinase: support yang digunakan adalah 1:1, 1,5:1, dan 2:1. Rasio 2:1 menghasilkan aktivitas enzim tertinggi, yaitu sebesar 2,030 ± 0,0405 U/ml. produksi NAG tertinggi diperoleh dari siklus fermentasi pertama yang menghasilkan konsentrasi NAG sebesar 1347,7778 ± 50,1848 ppm. Kitinase intraseluler yang diimobilisasi dengan alginat dapat digunakan hingga 4 siklus fermentasi dengan aktivitas enzim yang dipertahankan adalah sebesar 66,91%.
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