UJI AKTIVITAS ANTIOKSIDAN EKSTRAK BIJI DAN KULIT BUAH MELINJO (Gnetum gnemon L.)
Keywords:
antioxidant, flavonoid, melinjo, phenolicAbstract
Melinjo (Gnetum gnemon L.), widely cultivated in Southeast Asia, is known to have many phenolic and flavonoid compounds which have antioxidant activity. Antioxidants such as phenolic and flavonoids have photoprotective capabilities because they can help inhibit the formation of free radicals. In this research, seed and skin of melinjo were extracted using various solvents, such as hexane, ethyl acetate, and ethanol. Antioxidant activity test using DPPH, total flavonoid, and total phenolic content were tested for each fraction. The result showed that ethanol fraction of melinjo seed has the best antioxidant activity both for the seed and the skin. IC50 for ethanol fraction from skin is 440,58 ± 40,89 ppm with total flavonoid and phenolic content are 145,00 ± 23,79 QE/ g DW dan 32,31 ± 4,03 mg QE/ g DW. Meanwhile IC50 for ethanol fraction from seed is 424,78 ± 10,30 ppm with total flavonoid and phenolic content is 172,57 ± 18,01 mg QE/ g DW dan 32,31 ± 4,03 mg QE/ g DW.
References
Agustini, T. W., Suzery, M., Sutrisnanto, D., Ma’ruf, F. W., & Hadiyanto. 2015. Comparative study of bioactive substances extracted from fresh and dried Spirulina sp. Procedia Environmental Sciences 23: 282-289. Atun S, Arianingrum R, dan Masatake N. 2007. Some Phenolic Compounds From Stem Bark Of Melinjo (Gnetum gnemon) and Their Activity Test As Antioxidant And UV-B Protection. Proceeding JSChem-ITB-UKM. Kato, Eishin, Yuji Tokunaga, dan Fujio Sakan. 2009. Stilbenoids Isolated from the Seeds of Melinjo (Gnetum gnemon L.) and Their Biological Activity. Journal of Agricultural and Food Chemistry 57: 2544-2549. He S dan Yan X. 2013. From Resveratrol to Its Derivatives: New Sources of Natural Antioxidant. Curent Medicinal Chemistry Vol. 20 No. 4. Bentham Science Publishers. Manner, H.I. and Elevitch, C.R. 2006. “Gnetum gnemon (gnetum)”. Agroforestry online. Homepage on-line. Available from: http://www.agroforesty.net/tti/Gnetum-gnetum.pdf. Internet accessed 15 Mei 2012. Siswoyo TA. 2004. Physicochemical Characteristics of Melinjo (Gnetum gnemon) Starch-Lipid. Jurnal Ilmu Dasar, Vol. 5 No. 2.
Downloads
Published
Issue
Section
License
“Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC-BY-SA 4.0) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website). The final published PDF should be used and bibliographic details that credit the publication in this journal should be included.”