Rekayasa Genetika Metode CRISPR Cas9 dengan menggunakan Bakteri Escherichia coli dan Salmonella untuk Pengobatan Kanker; Genetic Engineering using the CRISPR-Cas9 Method using Esherichia coli and Salmonella Bacteria for the Treatment of Cancer

Krisdarwindah Mardiana, Margaret Theresia Hutagaol, Enjel Natanael Purba, Paldevina Br Maha, Wahyu Irawati

Abstract


Abstract

Cancer is a disease suffered by many people in Indonesia, so more modern technology is needed to treat cancer. The aim of this research is a genetic engineering system using the CRISPR-Cas9 method, through genetic engineering using Escherichia coli and Salmonella bacteria to treat cancer. This type of research is a literature review from research journals regarding genetic engineering to treat cancer using the CRISPR-Cas9 method. Escherichia coli bacteria and Salmonella bacteria can target cancer cells or hypoxic tumors.

Keywords: Escherichia coli bacteria; CRISPR-Cas9; DNA; cancer; genetic engineering

Abstrak

Penyakit kanker merupakan penyakit yang diderita oleh banyak orang di Indonesia sehingga dibutuhkan teknologi yang lebih modern untuk mengobati  penyakit kanker. Tujuan penelitian ini adalah pengertian sistem rekayasa genetika  menggunakan metode CRISPR-Cas9, susunan DNA pada bakteri E.coli dan bakteri  salmonella yang mendukung fungsinya dalam gen yang akan direkayasa, mekanisme sistem rekayasa genetika CRISPR-Cas9 dalam penyisipan kode gen, peranan CRISPR-Cas9 melalui rekayasa genetika melalui bakteri E.coli dan salmonella untuk mengobati penyakit kanker dan kelebihan dan kelemahan penggunaan bakteri E.coli dan salmonella dalam aplikasi metode CRISPR-Cas9. Jenis penelitian ini adalah bentuk deskriptif kualitatif dengan bentuk review dari jurnal hasil penelitian mengenai rekayasa genetika untuk mengobati penyakit kanker menggunakan metode CRISPR-Cas9. Aplikasi teknologi ini perlu mempertimbangkan dampak potensial dari pengobatan teknologi CRISPR/Cas bagi  keamanan penggunaannya karena teknologi ini dapat memodifikasi genom organisme  yang berdampak pada organisme non target.

Kata kunci: Escherichia coli, Salmonella sp., CRISPR-Cas9, DNA, rekayasa genetika


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